Cdk2 Antibody Cell Signaling

Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Cdk2 antibody cell signaling.

Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6. Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3. Lanes 1 3 and 5. Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.

Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Merged red and green signal.

Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160. Green signal from target ab32147 observed at 34 kda lanes 3 and 4. Kinase activity associated with cyclin a cdc2 is found only in g2 phase. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.

Lane 1 is 10 input. Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100. An antibody shouldn t be one of the variables in your experiment. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.

Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2. Recommended fluorochrome conjugated anti rabbit secondary antibodies. Find out why customers rank cst highest for antibody specificity and sensitivity. The antibody weakly cross reacts with cdc2 phosphorylated at thr161.

Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Inhibitory phosphorylation occurs on thr14 and tyr15 1.

Antibodies are purified by protein a and peptide affinity chromatography. Wild type hap1 cell lysate 20 µg lanes 2 4 and 6. Mix well then add 0 1 g bsa mix. Inhibitory phosphorylation occurs on thr14 and tyr15 1.

Western blot analysis was performed using cdk12 antibody. To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.